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Bone tissue engineering



Published on 19 August 2020
Objectives
Our initial studies showed that bone morphogenetic proteins (BMPs) can be presented to cells in a matrix-bound mannerr via a biomaterial surface (Crouzier et al, Small, 2009). This biomaterial is a biomimetic film, made by self-assembly of polysaccharides and polypeptides, which can trap the BMPs.
We then proved that 1) BMP-coated ceramic granules are osteoinductive in an ectopic site when implanted in rat muscle (Guillot et al, Biomaterials, 2011) 2) BMP-2-loaded film-coated titanium implants can be dried and sterlized while remaining osteoinductive (Guillot et al, Biomaterials, 2013) . In 2016, we showed that a critical size femoral bone defect in rats can be regenerated by combining an initially empty 3D polymeric scaffold made of to the osteoinductive film coating. The newly formed bone contained cortical and trabecular bones and it was vascularized (Bouyer et al, Biomaterials, 2016).
In collaboration with Prof. Georges Bettega (CHR Annecy and IAB Grenoble), we are continuing our pre-clinical developments in large animals in the frame of the ERC POC REGENERBONE (PI CPicart), Fondation Gueules Cassées and ANR OBOE 2019-2022 (PI CPicart).



Researchers and Technical Staff
Catherine Picart, CEA Research Director - Biography
Paul Machillot, CNRS Assistant Engineer - Biography

Students
Charlotte Garot (PhD student)
Michael Bouyer (PhD student)
Sanela Morand (M2 clinician)

Selected Publications
Ho-Shui-Ling A, Bolander J, Rustom LE, Johnsson AW, Luyten FP and Picart C

Bone regeneration strategies: Engineered scaffolds, bioactive molecules, and stem cells: current stage and future perspectives.
Biomaterials 2018, 180: 143-162

Kuterbekov M, Machillot P, Lhuissier P, Picart C, Jonas AM and Glinel K
Solvent-free preparation of porous poly(L-lactide) microcarriers for cell culture.
Acta Biomaterialia, 2018, 75: 300-311

Bouyer M, Guillot R, Lavaud J, Plettinx C, Olivier C, Curry V, Boutonnat J, Coll JL, Peyrin F, Josserand V, Bettega G and Picart C
Surface delivery of tunable doses of BMP-2 from a polymeric scaffold induces volumetric bone regeneration.
Biomaterials, 2016,104: 168-81

Guillot R, Pignot-Paintrand I, Lavaud J, Decambron A, Bourgeois E, Josserand V, Logeart-Avramoglou D, Viguier E and Picart C
Assessment of a polyelectrolyte multilayer film coating loaded with BMP-2 on titanium and PEEK implants in the rabbit femoral condyle.
Acta Biomaterialia, 2016, 36: 310–322

Monge C, Almodóvar J, Boudou T and Picart C
Spatio-temporal control of LbL films for biomedical applications: From 2D to 3D.
Advanced Healthcare Materials, 2015, 4(6): 811-830

Almodovar J, Guillot R, Monge C, Vollaire J, Seilimovic S, Coll JL, Khademhosseini A and Picart C
Spatial patterning of BMP-2 and BMP-7 on biopolymer films and the guidance of muscle cell fate.
Biomaterials, 2014, 35(13): 3975–3985

Guillot R, Gilde F, Becquart P, Sailhan F, Lapeyrere A, Logeart-Avramoglou D and Picart C
The stability of BMP loaded polyelectrolyte multilayer coatings on titanium.
Biomaterials, 2013, 34(23): 5737–5746

Gilde F, Maniti O, Guillot R, Mano JF, Logeart-Avramoglou D, Sailhan F and Picart C
Secondary structure of rhBMP-2 in a protective biopolymeric carrier material.
Biomacromolecules, 2012, 13(11): 3620–3626

Crouzier T, Sailhan F, Becquart P, Guillot R, Logeart-Avramoglou D and Picart C
The performance of BMP-2 loaded TCP/HAP porous ceramics with a polyelectrolyte multilayer film coating.
Biomaterials, 2011, 32(30): 7543-7554

Crouzier T, Fourel L, Boudou T, Albiges-Rizo C and Picart C
Presentation of BMP-2 from a soft biopolymeric film unveils its activity on cell adhesion and migration.
Advances Materials, 2011, 23(12): H111-H118

Crouzier T, Ren K, Nicolas C, Roy C and Picart C
Layer-by-layer films as a biomimetic reservoir for rhBMP2 delivery: Controlled differentiation of myoblasts to osteoblasts.
Small, 2009, 5(5): 598-608