​Par Jean-Christophe RAIN, Ph. D.
CEO Hybrigenics Services

Phage display nanobody selection from our fully synthetic humanized library ^[1] is a reality with more than a hundred successful projects since the publication of our library ^[2-7].
To complete our phage display selection of nanobodies, we took advantage from our 20 years of experience in Yeast genetics and developed both a Yeast 2-Hybrid platform and a Yeast display platform for optimization and intrabodization of nanobodies coming from our library or from the lama ^[7].
The selection and optimization of nanobodies and specifically nanobodies that could be expressed inside the cells (Intrabodies) will be illustrated with Nanobodies directed against TAU and providing anti-aggregative activity in vitro, in cellulo and in vivo in a mouse model of tauopathy.


1) Moutel S. et al. Elife 2016, 19: 5
2) Mikhaylova M, et al.Nat Commun., 2015 Aug 11;6: 7933
3) Galli V, et al. Elife, 2017 Oct 12; 6. pii: e25197
4) Jang H, et al. Mol Immunol. 2020 Feb;118: 110-116
5) Elorza-Vidal X et al. Hum Mol Genet. 2020 May 8;29(7): 1107-1120
6) Kawecki C, et al. J Thromb Haemost. 2020 Jun 4.
7) Dupré E, et al. ACS Chem Neurosci. 2019 Sep 18;10(9): 3997-4006