3D cell culture (Prostate cancer, Breast cancer)
Published on 20 August 2020
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Project leader
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Nathalie Picollet-D'hahan Laboratoty of Biology and Biotechnology for Health Biomics team 17 avenue des Martyrs 38 054 Grenoble cedex 09 Phone: 33 (0)4 38 78 67 78 Fax: 33 (0)4 38 78 59 17 |
3D Organoids: From organogenesis to carcinogenesis
We develop
3D model models (Figure 1) to understand the determinants that regulate normal development of
prostatic tissue and how they may be disrupted in disease states such as
cancer The originality of our research is to
engineer and study prostate and breast organoids by using
microfluidics (Figure 2) and innovative
3D lens-free imaging in collaboration with CEA/LETI. We aim at providing new insights in the mechanisms controlling epithelial differentiation and polarity of glandular tissues. To do so, RNAi-based screening approaches are performed to address issues in development and carcinogenesis.
Picollet-D'hahan N, Dolega ME, Liguori L, Marquette C, Le Gac S, Gidrol X and Martin DK
A 3D toolbox to enhance physiological relevance of human tissue models.
Trends in Biotechnology, 2016,
34(9): 757-769
Dolega ME, Abeille F, Picollet-D’hahan N and Gidrol X
Controlled 3D culture in Matrigel microbeads to analyze clonal acinar development.
Biomaterials, 2015,
52: 347-357
Organ-on-chip technology: New insights in exocrine function
Organs-on-chips are becoming a powerful technology for creating novel human organ and disease models. Our
prostate-on-chip devices are developed by growing three-dimensional living cells in a dynamic microfluidic ‘chip’ environment and supported by
3D free-standing scaffolds (collaboration UGA/TIMC and 3d.fab platform of Lyon). Such organ-on-chip models mimic prostate or breast 3D architectures and function and support secretomics-based diagnosis and flow-based drug screening (Figure 3).
Picollet-D'hahan N, Gerbaud S, Kermarrec F, Alcaraz JP, Obeid P, Bhajun R, Guyon L, Sulpice E, Cinquin P, Dolega ME, Wagh J, Gidrol X and Martin DK
The modulation of attachment, growth and morphology of cancerous prostate cells by polyelectrolyte nanofilms.
Biomaterials, 2013,
34(38): 10099-10108
1654215 FR; 1654213 FR
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